Can I inseminate my dog at home? - Veterinary Village
Actually, you can inseminate your dog at home if you choose to do vaginal insemination. Anything more sophisticated than that requires a veterinarian's assistance, of course, but vaginal AIs are allowed. In fact, a few years ago the AKC determined that clients are allowed to do their own vaginal inseminations and still register the litter as an assisted semen breeding. So yes, you can do them as long as you're using fresh semen. Frozen semen should never be put in any way other than with transcervical or surgical because the success rate of inseminating frozen semen vaginally is about 11% as opposed to about 80% when it's put into the uterus directly.
Contributed by Marty Greer DVM, JD
from Veterinary Village